P8203

PIPES

BioXtra, ≥99% (titration)

• 동의어(들): 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
• 실험식(Hill 표기법): C₈H₁₈N₂O₆S₂
• CAS Number: 5625-37-6
• Molecular Weight: 302.37
• Beilstein: 817713
• EC Number: 227-057-6
• MDL number: MFCD00006159
• UNSPSC 코드: 12161700
• PubChem Substance ID: 24898917
• NACRES: NA.25

속성

• 제품 라인: BioXtra
• Quality Level: 400
• 분석: ≥99% (titration)
• 양식: powder
• 불순물: Insoluble matter, passes filter test
• 무기 잔류물(900 °C): ≤0.5% (as SO₄)
• 손실: ≤0.5% loss on drying, 110°C
• 유용한 pH 범위: 6.1-7.5
• pKa(25 °C): 6.8
• mp: >300 °C (lit.)
• solubility: 1 M NaOH: 0.5 M at 20 °C, clear, colorless
• 음이온 미량물: chloride (Cl^-): ≤0.2%
• 양이온 미량물: Al: ≤0.005%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.005%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.1%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• 흡수: ≤0.1 at 260 in 1 M NaOH at 0.5 M; ≤0.1 at 280 in 1 M NaOH at 0.5 M
• 응용 분야: diagnostic assay manufacturing
• SMILES string: OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
• InChI key: IHPYMWDTONKSCO-UHFFFAOYSA-N

설명

일반 설명

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al.,[1] developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

애플리케이션

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols.[2] PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.[3]^,[4]
PIPES has been utilized in protein crystallization.[5]^,[6] The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described.[7] PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.[8]
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays.[9] PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.[10]

품질

Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.

결합

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation.

제조 메모

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)