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P1851

PIPES

BioPerformance Certified, suitable for cell culture

Synonym(s):

1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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Pack SizeSKUAvailabilityPrice
25 g

Available to ship TODAYfromMILWAUKEE

$44.90
100 g

Available to ship TODAYfromMILWAUKEE

$141.00
500 g

Available to ship TODAYfromMILWAUKEE

$571.00
1 kg

Available to ship TODAYfromMILWAUKEE

$1,010.00

About This Item

Empirical Formula (Hill Notation):
C8H18N2O6S2
CAS Number:
Molecular Weight:
302.37
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
227-057-6
Beilstein/REAXYS Number:
817713
MDL number:

$44.90


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Quality Level

grade

BioPerformance Certified, Molecular Biology

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and Total Aerobic Microbial Count, tested

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless, soluble

application(s)

diagnostic assay manufacturing

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This Item
P6757P820380635
grade

BioPerformance Certified, Molecular Biology

grade

-

grade

-

grade

Molecular Biology

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

form

crystalline powder

form

crystalline powder

form

powder

form

powder

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless, soluble

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

Application


  • Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics.: The use of PIPES buffer in this study was integral to maintaining the stability of immunoglobulin G during differential scanning fluorimetry. This method is crucial for evaluating the stability of antibody-based therapeutics (Moggridge et al., 2017).

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Veronique Adoue et al.
Molecular systems biology, 10, 754-754 (2014-10-19)
Most complex disease-associated genetic variants are located in non-coding regions and are therefore thought to be regulatory in nature. Association mapping of differential allelic expression (AE) is a powerful method to identify SNPs with direct cis-regulatory impact (cis-rSNPs). We used
Leticia A Montoya et al.
Analytical chemistry, 86(12), 6032-6039 (2014-05-24)
Sulfhydryl-containing compounds, including thiols and hydrogen sulfide (H2S), play important but differential roles in biological structure and function. One major challenge in separating the biological roles of thiols and H2S is developing tools to effectively separate the reactivity of these
V D Prokopieva et al.
Alcohol and alcoholism (Oxford, Oxfordshire), 35(1), 44-48 (2000-02-24)
The effects of carnosine and related compounds on erythrocytes from alcoholics were studied. In their presence, erythrocytes showed an increased ability to resist haemolysis and showed a more normal morphology, with carnosine and N-acetyl-carnosine being the most effective compounds. These
B W Nielsen et al.
Agents and actions, 35(3-4), 170-178 (1992-03-01)
Increased osmotic pressure has been reported to cause non-cytotoxic histamine release (HR) from human basophils, as well as a potentiation of HR induced by anti-IgE. In this study, the effects of hyperosmolar Na-K-acetate (300-600 mOsm/kg H2O) on HR was studied
M Le Hir
Enzyme, 45(4), 194-199 (1991-01-01)
An impurity, probably an anion, present in some batches of the buffer substances 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), 2-morpholinoethane sulfonic acid (Mes) and piperazine-1,4-bis(2-ethane sulfonic acid (Pipes), activates the soluble 5'-nucleotidase from rat kidney. The affinity of the enzyme for 5'-IMP

Global Trade Item Number

SKUGTIN
P1851-100G04061834356837
P1851-1KG04061826757512
P1851-25G04061834356905
P1851-500G04061834356912

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, I bought PIPES and HEPES powder for a buffer solution. Should I store those at room temperature or fridge (4C)? Thank you Andrea

    1 answer
    1. In powder form, both the PIPES and HEPES may be stored tightly sealed at room temperature.

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