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Merck
모든 사진(1)

주요 문서

X100

Sigma-Aldrich

Triton X-100

Triton™ X-100

laboratory grade

동의어(들):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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크기 선택

5 ML
₩35,525
100 ML
₩106,474
500 ML
₩136,010
1 L
₩192,140
1 GAL
₩506,688
5 GAL
₩1,602,178

₩35,525


재고 있음세부사항


벌크 견적 요청

크기 선택

보기 변경
5 ML
₩35,525
100 ML
₩106,474
500 ML
₩136,010
1 L
₩192,140
1 GAL
₩506,688
5 GAL
₩1,602,178

About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
UNSPSC 코드:
12161900
PubChem Substance ID:
NACRES:
NA.21

₩35,525


재고 있음세부사항


벌크 견적 요청

생물학적 소스

synthetic

Quality Level

Grade

laboratory grade

설명

non-ionic

양식

liquid

mp

6  °C (43 °F)

전이 온도

cloud point 65 °C
pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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일반 설명

Triton X-100 has hemolytic property[1] and is used for DNA extraction.[2]
Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[3][4]

애플리케이션

Triton-X has been used:
  • In the permeabilization of cells for immunofluorescence staining[5]
  • As a component of lysis buffer in western blot analysis[5]
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope[5]

생화학적/생리학적 작용

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

특징 및 장점

  • Ideal for Cell Biology and Biochemical research applications
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

픽토그램

CorrosionExclamation markEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

483.8 °F - closed cup

Flash Point (°C)

251 °C - closed cup

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

이미 열람한 고객

Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)
José Francisco Lima-Barbero et al.
Experimental & applied acarology, 78(4), 555-564 (2019-08-02)
Blood-feeding ectoparasites constitute a growing burden for human and animal health, and animal production worldwide. In particular, mites (Acari: Gamasida) of the genera Dermanyssus (Dermanyssidae) and Ornithonyssus (Macronyssidae) infest birds and cause gamasoidosis in humans. The tropical fowl mite, Ornithonyssus
Knockdown of miR-210 decreases hypoxic glioma stem cells stemness and radioresistance
Yang W, et al.
Experimental Cell Research, 326(1), 22-35 (2003)
alpha -Synuclein Is Degraded by Both Autophagy and the Proteasome
Webb LJ, et al.
The Journal of Biological Chemistry, 278(27), 25009-25013 (2003)
Hiroki Sakamoto et al.
Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs, 22(3), 222-229 (2019-05-12)
In recent years, studies on liver graft construction using the decellularized liver as a template for transplantation therapy have attracted much attention. However, the therapeutic effect of constructed liver grafts in hepatic failure has not been evaluated. Therefore, we aimed

문서

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

프로토콜

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

This page shows how to solubilize membrane proteins with products from Cytiva.

질문

1–10 / 16 질문  
  1. How long is Triton X-100 stable at -80 degrees in buffer?

    1 답변
    1. At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.

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  2. If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?

    1 답변
    1. Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
      concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
      https://www.sigmaaldrich.com/product/sigma/t8787
      https://www.sigmaaldrich.com/product/mm/648466
      https://www.sigmaaldrich.com/product/sigma/93443

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 답변
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  4. How can I determine the shelf life / expiration / retest date of this product?

    1 답변
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  5. What is the correct CAS number for Triton X-100, 9002-93-1 or 9036-19-5?

    1 답변
    1. CAS 9036-19-5 is a generic CAS number and is correct for Triton® X-100. With this number, neither the substitution pattern of the aromatic ring (para, meta, ortho), nor the amount of the repeating units of the oxyethylene units is specified or described. Triton® X-100 with CAS 9002-93-1 is also correct but it defines only the para-substitution.

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  6. Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?

    1 답변
    1. Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.

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  7. Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?

    1 답변
    1. The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.

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  8. What are the NFPA hazard numbers for this?

    1 답변
    1. With the implementation of the Globally Harmonized System (GHS) of hazard classification in our safety data sheets (SDS), we decided to no longer maintain the National Fire Protection Association (NFPA) information in section 15 of the SDS. Therefore, we no longer provide updates to the NFPA ratings. Kindly review the SDS available at this link: https://www.sigmaaldrich.com/US/en/sds/sial/x100?userType=anonymous

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  9. Hi, Bought the X100-100ml but cant find what the concentration is. Is this 100%? Kind regards,

    1 답변
    1. The product is a neat or pure liquid, the concentration is 100 %.

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  10. What's the molar concentration of the TritonX 100?? How can I make a 50mM concentration from this??

    1 답변
    1. This product is supplied as a neat or pure liquid with a density of 1.07 g/mL and an average molecular weight of 625 g/mol. Assuming 100%, the molarity is 1.712 M. To make 1 L of a 50 mM solution from this, use 29.2 mL of the 1.712 M starting concentration.

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1–10 / 16 질문  

후기

활성 필터

  1. Lillehammer, Norway
    • 상품평 2
    • 투표 1
    별 5개 중 5개입니다.

    M.Sc.

    We use the Product for surface cleaning of artworks. Works as planned.

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  2. 1 평점-상품평만

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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