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Merck
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문서

P5472

Sigma-Aldrich

Precast Agarose Gels

8-well (portrait orientation)

동의어(들):

Precast agarose gels for high resolution nucleic acids separation

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About This Item

UNSPSC 코드:
41105300
NACRES:
NB.22

Grade

for molecular biology

형태

gel form

포함

0.5 μg/mL ethidium bromide

저장 온도

room temp

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일반 설명

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style

애플리케이션

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.

TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.

MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Suitable for separation of DNA fragments ranging from 500 - 1000 base pairs.

포장

Package of 20 individually foil-wrapped gels.

성분

Precast gels contain 1.0% agarose in 1X TBE buffer with 0.5 ug/ml ethidium bromide.

기타 정보

DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)

품질

No detected DNase or RNase activity

물리적 형태

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)

관련 제품

제품 번호
설명
가격

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서

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프로토콜

These gels are suitable for separating nucleic acids, giving sharp DNA bands and low background fluorescence.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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