추천 제품
생물학적 소스
algae (red)
형태
powder
기술
electrophoresis: suitable
불순물
≤10% water
EEO
≤0.10
전이 온도
gel point 38-43 °C (10% gel)
겔 강도
≥2000 g/cm2
음이온 미량물
sulfate (SO42-): ≤0.20%
외래 활성
DNase, RNase, NICKase, none detected
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
유사한 제품을 찾으십니까? 방문 제품 비교 안내
특이성
Suitable for the separation of high molecular weight DNA. Gels are easy to handle and give faster separation and better resolution of high molecular weight DNA by field inversion electrophoresis than our routine-use agarose.
애플리케이션
Especially formulated for fast resolution of large DNA (10-2000 ·kb) by pulsed-field gel electrophoresis (PFGE) or conventional electrophoresis. Pulsed-field gel electrophoresis was developed in 1984 to deal with "reptation" of large DNA′s whose size surpass the limit of resolution and all migrate at the same rate. Alternately pulsed, orthogonally oriented electric fields are applied across the gel. Every time the direction of the electric field changes, the large DNA′s can no longer migrate until they become reoriented along the new electric field. The time required for reorientation is dependent on the size of the DNA, therefore the DNA is fractionated by size.
특징 및 장점
- Low EEO allows for increased voltages and more rapid DNA migration
- Greater gel strength allows for stability of lower concentration gels leading to faster DNA migration
- Gels exhibit low background fluorescence using ethidium bromide staining
분석 메모
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Sifang Liao et al.
Frontiers in endocrinology, 11, 180-180 (2020-05-07)
The insulin/IGF-signaling pathway is central in control of nutrient-dependent growth during development, and in adult physiology and longevity. Eight insulin-like peptides (DILP1-8) have been identified in Drosophila, and several of these are known to regulate growth, metabolism, reproduction, stress responses
Yu-Hsuan Cheng et al.
BMC biology, 18(1), 180-180 (2020-12-01)
Ciliates are an ancient and diverse eukaryotic group found in various environments. A unique feature of ciliates is their nuclear dimorphism, by which two types of nuclei, the diploid germline micronucleus (MIC) and polyploidy somatic macronucleus (MAC), are present in
Sujith V W Weerasinghe et al.
Journal of medicinal chemistry, 51(18), 5542-5551 (2008-08-30)
Histone deacetylase 1 (HDAC1) has been linked to cell growth and cell cycle regulation, which makes it a widely recognized target for anticancer drugs. Whereas variations of the metal-binding and capping groups of HDAC inhibitors have been studied extensively, the
Michael J Lafferty et al.
The Journal of biological chemistry, 288(40), 28524-28534 (2013-08-21)
Elevated triglycerides are associated with an increased risk of cardiovascular disease, and lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of triglycerides from circulating lipoproteins. The N-terminal domain of angiopoietin-like protein 4 (ANGPTL4) inhibits LPL activity. ANGPTL4 was
Julia Dahlmann et al.
Biomaterials, 34(10), 2463-2471 (2013-01-22)
In most pluripotent stem cell differentiation protocols the formation of embryoid bodies (EBs) is an important step. Here we describe a rapid, straightforward soft lithography approach for the preparation of hydrophilic silicon masters from different templates and the subsequent production
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.