S8140

SSPE Buffer

for Northern and Southern blotting, powder blend

• 동의어(들): Saline-Sodium Phosphate-EDTA Buffer
• UNSPSC 코드: 41105319
• NACRES: NA.25

속성

• Grade: for molecular biology
• 형태: powder blend
• pH: 7.2-7.6 (20 × in water, high purity, ambient)
• solubility: water, high purity: 20 ×, clear, colorless
• 외래 활성: DNAse, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; RNAse, none detected
• 저장 온도: room temp

설명

일반 설명

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

애플리케이션

• Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).


• Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

특징 및 장점

• Easy to reconstitute powder
• Multi-purpose use in hybridizations

성분

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges