M2154

Medium 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

• 동의어(들): M199 Medium, TCM 199
• UNSPSC 코드: 12352207
• NACRES: NA.75

속성

• product name: Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• 무균: sterile-filtered
• 형태: liquid
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• pH: 7.0-7.6 (25 °C, 209 g/L)
• 성분: sodium pyruvate: no; L-glutamine: no; NaHCO₃: yes; HEPES: no; phenol red: yes; Earle’s salts (5% CO₂): yes
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

애플리케이션

Medium 199 has been used:

• to culture rumen epithelial cells from yearling Speckle Park beef heifers
• as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries
• as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)

재구성

Supplement with 0.1 g/L L-glutamine.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable