product M1255, Calcein Blue to is used to stain cells, but it will stain living or dead cells. For a cell viability stain, one can use Calcein Blue AM. The difference between Calcein Blue and Calcein Blue AM is the methyl-iminodiaceitc acid group that chelates the divalent metal has acetoxymethyl esters on it in Calcein Blue AM. The esters are cleaved inside of viable cells, but remain on the molecule in the absence of active esterases. So, if you use M1255 it will stain all the cell whether alive or dead.
모든 사진(3)
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보기 변경
1 G
₩145,653
10 G
₩781,753
About This Item
실험식(Hill 표기법):
C15H15NO7
CAS Number:
Molecular Weight:
321.28
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47
추천 제품
제품명
Calcein Blue, fluorescent dye
양식
powder
기술
titration: suitable
색상
white to light yellow
solubility
1 M NaOH: 50 mg/mL
형광
λex 322 nm; λem 445 nm in methanol
적합성
suitable for indicator for EDTA titration
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
InChI
1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
InChI key
RJPSHDMGSVVHFA-UHFFFAOYSA-N
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일반 설명
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
애플리케이션
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
개인 보호 장비
dust mask type N95 (US), Eyeshields, Gloves
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Indicators: International Series of Monographs in Analytical Chemistry (2013)
T Lymphocytes Influence the Mineralization Process of Bone.
El Khassawna T
Frontiers in Immunology, 8, 562-562 (2017)
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Aging, 8(11), 2871-2896 (2016-11-18)
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문서
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