C1359

Calcein AM solution

≥90% purity (HPLC), solution

• 실험식(Hill 표기법): C₄₆H₄₆N₂O₂₃
• CAS Number: 148504-34-1
• Molecular Weight: 994.86
• EC Number: 200-664-3
• MDL number: MFCD05861516
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24892405
• NACRES: NA.47

속성

• product name: Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
• Quality Level: 100
• 분석: ≥90% (HPLC)
• 형태: solution
• 농도: 4 mM in DMSO
• 색상: colorless; white to off-white
• λmax: 491-497 nm
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: −20°C
• SMILES string: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• InChI key: XKFSBWQWNMZWFA-UHFFFAOYSA-N

설명

애플리케이션

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

생화학적/생리학적 작용

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Fluorescent cell permeable derivative of calcein.

물리적 형태

Solution in 0.4% Calcein AM and 99.6% DMSO.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 185.0 °F - closed cup
• Flash Point (°C): 85 °C - closed cup
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)