17783

Calcein-AM

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

• 동의어(들): Calcein acetoxymethyl ester, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
• 실험식(Hill 표기법): C₄₆H₄₆N₂O₂₃
• CAS Number: 148504-34-1
• Molecular Weight: 994.86
• MDL number: MFCD05861516
• UNSPSC 코드: 12171500
• PubChem Substance ID: 57647670
• NACRES: NA.32

속성

• 제품 라인: BioReagent
• 분석: ≥95.0% (HPLC)
• 형광: λ_ex 496 nm; λ_em 516 nm in 0.1 M Tris pH 8.0, esterase; Ca²⁺
• 적합성: suitable for fluorescence
• 저장 온도: −20°C
• SMILES string: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• InChI key: XKFSBWQWNMZWFA-UHFFFAOYSA-N

설명

일반 설명

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

애플리케이션

Calcein-AM has been used in flow cytometry.

Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)