LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
L3272
LB Broth with agar (Luria low salt)
Powder microbial growth medium
동의어(들):
Luria Agar Base (Miller’s Modification)
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1 KG
₩349,465
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About This Item
UNSPSC 코드:
41106200
NACRES:
NA.85
₩349,465
구입 가능 여부는 고객센터에 문의하십시오.
기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요
추천 제품
Grade
Molecular Biology
for molecular biology
Quality Level
무균
non-sterile
양식
powder
구성
Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
기술
microbiological culture: suitable
pH
6.8-7.2(3.05% solution)
응용 분야
food and beverages
microbiology
저장 온도
room temp
적합성
nonselective for Escherichia coli
nonselective for coliforms
일반 설명
Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
애플리케이션
LB Broth with agar (Luria low salt) has been used:
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
특징 및 장점
Luria′s low salt LB powder with agar provides:
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
제조 메모
1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
법적 정보
Zeocin is a registered trademark of Cayla Sarl
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
Difco Manual, 271-271 (1998)
Increased Expression of a Reporter Plasmid by Crotalid Venom Fraction
Islas AA, et al.
Journal of Animal and Veterinary Advances, 9(17), 2214-2219 (2010)
Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth
Grobelak A, et al.
Ecological Engineering, 84, 22-28 (2015)
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
Vidya Padmanabhan Nair et al.
STAR protocols, 3(2), 101281-101281 (2022-04-26)
Human endogenous retroviruses (HERVs) comprise many regulatory elements and can regulate host gene activity at different expression levels via multiple mechanisms. Here, we introduce a step-by-step protocol to activate or repress transcription of HERV-K(HML-2) elements using the CRISPRa and CRISPRi
프로토콜
General protocols for growth of competent cells in microbial medium.
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 답변-
도움이 되었습니까?
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Will adding magnesium to the culture medium increase cell density?
1 답변-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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Which bacterial culture medium is the best choice for my application?
1 답변-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
도움이 되었습니까?
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What is the difference between the LB - Luria low salt products?
1 답변-
LB - Luria low salt is available in both powder (L3397) and powder with agar formats (L3272).
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