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Merck
모든 사진(2)

문서

L3022

Sigma-Aldrich

LB Broth (Lennox)

Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

동의어(들):

Lennox broth, Lennox broth , Luria Bertani Broth

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About This Item

UNSPSC 코드:
41106200
NACRES:
NA.85

Grade

for molecular biology

무균

non-sterile

형태

powder

구성

NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

기술

microbiological culture: suitable

pH

6.8-7.2(2% solution)

응용 분야

food and beverages
microbiology

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

일반 설명

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

애플리케이션

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
It was used for resuspension of T. pyriformis cell fractions in a study of Trimastix fractionation of cellular extracts.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

특징 및 장점

Lennox LB powder provides:
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to liquid
  • Standard formulation

제조 메모

1. Suspend 20 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

법적 정보

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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프로토콜

General protocols for growth of competent cells in microbial medium.

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