F9037

Formamide

BioReagent, ≥99.5% (GC), for molecular biology

• 동의어(들): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• EC Number: 200-842-0
• MDL number: MFCD00007941
• UNSPSC 코드: 12352111
• PubChem Substance ID: 24894985
• NACRES: NA.52

속성

• Grade: for molecular biology
• Quality Level: 200
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• 제품 라인: BioReagent
• 분석: ≥99.5% (GC)
• 형태: liquid
• autoignition temp.: 932 °F
• 기술: electrophoresis: suitable; immunohistochemistry: suitable
• refractive index: n20/D 1.447 (lit.)
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• density: 1.134 g/mL at 25 °C (lit.)
• 적합성: suitable for nucleic acid hybridization
• 저장 온도: 2-8°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

설명

일반 설명

Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.

애플리케이션

Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other applications include:

• Preparation of brain sections of mice for BrdU immunohistochemistry
• FISH analysis of HeLa cells.
• Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

특징 및 장점

• Deionized for molecular biology applications
• Packaged under argon gas in amber bottles to protect from oxidation
• Used for DNA renaturation or DNA-RNA hybridization
• Reduces thermal stability of double stranded nucleic acids

적합성

• nucleic acid hybridization
• cryoprotectant and gel-stabilizer
• destabilize nucleic acid duplexes
• elimination of secondary structure of nucleic acids
• preparation of hybridization buffers for RNA and DNA application

기타 정보

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H351,H360D,H373
• 예방조치 성명서: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• 표적 기관: Blood
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): 305.6 °F
• Flash Point (°C): 152 °C
• 개인 보호 장비: Eyeshields, Gloves, type ABEK (EN14387) respirator filter