47671

Formamide

BioUltra, for molecular biology, ≥99.5% (T)

• 동의어(들): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• EC Number: 200-842-0
• MDL number: MFCD00007941
• UNSPSC 코드: 12352111
• PubChem Substance ID: 57651075
• NACRES: NA.25

속성

• Grade: for molecular biology
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• 제품 라인: BioUltra
• 분석: ≥99.5% (T)
• 형태: liquid
• autoignition temp.: 932 °F
• 기술: FISH: suitable; hybridization: suitable; protein extraction: suitable
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• 무기 잔류물: ≤0.1%
• refractive index: n20/D 1.447 (lit.); n20/D 1.447
• pH: 7-9 (25 °C, 1 M in H₂O)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• solubility: H₂O: 10 M at 20 °C, clear, colorless
• density: 1.134 g/mL at 25 °C (lit.)
• 음이온 미량물: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• 양이온 미량물: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤1000 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• SMILES string: NC=O
• λ: 10 M in H₂O
• UV 흡수: λ: 260 nm A_max: ≤0.4; λ: 280 nm A_max: ≤0.1
• 응용 분야: genomic analysis
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

설명

일반 설명

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

애플리케이션

Formamide has been used:

• as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
• in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
• in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
• to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
• as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

생화학적/생리학적 작용

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

특징 및 장점

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

기타 정보

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For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H351,H360D,H373
• 예방조치 성명서: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• 표적 기관: Blood
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): 305.6 °F
• Flash Point (°C): 152 °C
• 개인 보호 장비: Eyeshields, Gloves, type ABEK (EN14387) respirator filter