P4557

Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

• 동의어(들): Tris-equilabrated Phenol
• 실험식(Hill 표기법): C₆H₆O
• CAS Number: 108-95-2
• Molecular Weight: 94.11
• Beilstein: 6428463
• EC Number: 203-632-7
• MDL number: MFCD00002143
• UNSPSC 코드: 12352104
• PubChem Substance ID: 57654529
• NACRES: NA.52

속성

• Grade: for molecular biology
• 제품 라인: BioReagent
• 분석: ≥99.0% (GC)
• 형태: liquid
• pH: 7.7-8.1 (25 °C)
• 저장 온도: 2-8°C
• SMILES string: Oc1ccccc1
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• 유전자 정보: human ... GABRA1(2554)

설명

일반 설명

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

애플리케이션

Phenol solution has been used:

• as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
• for extraction of DNA from Arabidopsis thaliana Rosette leaves
• in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
• as a component of extraction buffer to extract RNA from fruit pulp
• for the extraction of protein from roots
• for the isolation of fecal DNA
• for the extraction of caged RNA

생화학적/생리학적 작용

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.

성분

• 1 bottle Tris-equilabrated Phenol (product number P4557)
• Equilibration Buffer (product B5658)

안전성 정보

• 픽토그램: GHS06,GHS08,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H301 + H311 + H331,H314,H341,H373,H411
• 예방조치 성명서: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• 표적 기관: Nervous system,Kidney,Liver,Skin
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter