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Merck
모든 사진(1)

주요 문서

H7140

Sigma-Aldrich

Hybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

UNSPSC 코드:
12161700
NACRES:
NA.25

Quality Level

양식

liquid (2x concentrate)

외래 활성

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

저장 온도

−20°C

일반 설명

Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

애플리케이션

Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

성분

Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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프로토콜

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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