This product has not undergone testing to determine the presence of dopamine or serotonin.
F7524
Fetal Bovine Serum
Fetal Bovine Serum
1명의 제품 평가자(총 8명)가 샘플 제품을 받았거나 프로모션에 참여했습니다
non-USA origin, sterile-filtered, suitable for cell culture
동의어(들):
Fetal Calf sera, Fetal Calf serum, FBS, FCS, sera, serum
로그인조직 및 계약 가격 보기
모든 사진(1)
About This Item
생물학적 소스:
bovine fetus
기원:
non-USA origin
무균:
sterile-filtered
배송 상태:
dry ice
가격 및 재고 정보를 현재 이용할 수 없음 고객지원팀으로 연락바랍니다.
추천 제품
애플리케이션
Fetal Bovine Serum has been used as a supplement in cell culture media such as Roswell Park Memorial Institute medium (RPMI-1640) and Dulbecco′s Modified Eagles Media (DMEM)[1]to support the growth of a variety of cell lines.
생화학적/생리학적 작용
Fetal bovine serum (FBS) serves as a commonly utilized cell culture media supplement to support in vitro and ex vivo cell, tissue, and organ cultures. FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth, and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth-promoting and survival enhancing factors to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. FBS is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
특징 및 장점
F7524 is the same great product as Biochrom GmbH. product S0115.
분석 메모
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
법적 정보
Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.
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또한 이 제품과 함께 일반적으로 구입
제품 번호
설명
가격
추천
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Hans F N Kvitvang et al.
Journal of chromatography. A, 1370, 70-79 (2014-12-03)
Metabolic profiling has become an important tool in biological research, and the chromatographic separation of metabolites coupled with mass spectrometric detection is the most frequently used approach for such studies. The establishment of robust chromatographic methods for comprehensive coverage of
Elisabeth F P Peterse et al.
BMC cancer, 16, 475-475 (2016-07-16)
Chondrosarcoma is a malignant cartilage forming bone tumour for which no effective systemic treatment is available. Previous studies illustrate the need for a better understanding of the role of the IGF pathway in chondrosarcoma to determine if it can be
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Maria Peiris-Pagès et al.
Aging, 11(14), 4801-4835 (2019-07-18)
Using proteomics analysis, we previously compared MCF7 breast cancer cells grown as 3D tumor spheres, with the same cell line grown as monolayers. Our results indicated that during 3D anchorage-independent growth, the cellular machinery associated with i) mitochondrial biogenesis and
문서
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
관련 콘텐츠
머크의 포괄적인 우태혈청(FBS) 포트폴리오에서 귀하의 실험실 요건과 예산에 적합한 제품을 찾아보십시오. 지구촌의 문제가 FBS 가격에 미치는 영향에 대해 알아보십시오.
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
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Hello, I have a question regarding FBS (F7524), for my experiment it is essential there is no dopamine or serotonine in my medium. Is it known wether these substances are present in FBS?
1 답변-
도움이 되었습니까?
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Could the presence of insoluble precipitates when thawing the serum indicate contamination?
1 답변-
Thawed serum may contain precipitates and flocculants due to various reasons, such as residual fibrinogen converting to fibrin upon thawing. Centrifugation at 400 xg can remove excess fibrin, while filtering serum is not recommended to avoid protein depletion and clogged filters.
Additional points to consider:
•Cloudiness in human serum products is common and indicates the presence of lipids. Clear, lipid-free human serum products are not available.
•Microscopic serum precipitates may resemble yeast contamination but are actually lipid aggregates that can be dissolved by lipase. They do not show growth in culture or gram staining and can be removed through centrifugation or filtration.
•Gel layer or clumps in serum are caused by poor or overheated inactivation or improper mixing of FBS during the Heat inactivation process. Serum with these characteristics should not be used.
•To minimize denaturation of serum lipoproteins and turbidity, it is recommended to prepare single-use aliquots and avoid repeated freeze-thaw cycles.도움이 되었습니까?
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Is this product F7524 heat-inactivated? Should it be prior to use?
1 답변-
This product has not been heat inactivated. For most cell culture applications heat inactivation is not necessary. If the application requires inactivation of any heat labile serum components, such as compliment or cytokines, the product may be heat inactivated in the lab. Alternatively, a heat inactivated version of this product is also available. See product F9665.
To heat inactivate this product:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Avoid repeated thaw-freeze cycles.도움이 되었습니까?
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Bonjour, Quelle est la composition et la concentration du sérum référencé F7524 en Calcium ? Cordialement
1 답변-
Calcium levels are not determined for this product. The serum is a natural product that will contain calcium at physiological levels.
도움이 되었습니까?
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Welches fetale Kälbserum wurde für die Kryokonservierung von Mausembryonen oder Eiestockmäus verwendet?
1 답변-
Unfortunately, a fetal calf serum that has been specifically tested for cryopreservation of mouse embryos or ovarian mice is not currently offered. Please see the link below to review Product ES009-M, Fetal Bovine Serum. This product is tested for expansion and cytotoxicity of mouse embryonic stem cells and may be of interest:
https://www.sigmaaldrich.com/product/mm/es009m도움이 되었습니까?
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How can we heat inactivate the fetal bovine serum cat: F7524? For how long?
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All heat inactivated serum products are treated at 56 deg. C for 30 minutes. This process may be applied to manually heat inactivate serum on a per bottle basis using a water bath. When immersing bottles do not allow water to come in contact with the cap/closure.
도움이 되었습니까?
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Is Feta bovine serum F7524-500ML heat inactivated?
1 답변-
This product is not heat-inactivated.
도움이 되었습니까?
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Is Product F7524, Fetal Bovine Serum, a USDA approved serum?
1 답변-
This is a non-USDA approved serum. The serum is tested for BVD (bovine virus diarrhea), PI3 (parainfluenza type 3), and IBR (infectious bovine rhinotracheitis) only.
도움이 되었습니까?
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Where is Product F7524, Fetal Bovine Serum, sourced from?
1 답변-
Product is South American Origin and EU approved. Some batches could be sourced from France.
도움이 되었습니까?
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How is Product F7524, Fetal Bovine Serum, sterilized?
1 답변-
This product is sterilized by filtration through three (3) sterile filter membranes, each of which has a pore size of 0.1 microns.
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