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Merck
모든 사진(1)

문서

F4042

Sigma-Aldrich

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

동의어(들):

Anti-ddddk, Anti-dykddddk

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모든 사진(1)

About This Item

MDL number:
MFCD00282246
UNSPSC 코드:
12352203
NACRES:
NA.32

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

M5, monoclonal

형태

buffered aqueous solution

정제법

using Protein A

종 반응성

all

농도

2-5 mg/mL

기술

western blot (chemiluminescent): 10 μg/mL

동형

IgG1

면역원 서열

DYKDDDDK

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

면역원

FLAG; peptide sequence DYKDDDDK

애플리케이션

Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

물리적 형태

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

제조 메모

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

기타 정보

Antibody is not calcium dependent.

법적 정보

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

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