추천 제품
생물학적 소스
rat
결합
unconjugated
항체 형태
purified from hybridoma cell culture
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
6F7, monoclonal
양식
buffered aqueous solution
종 반응성
all
기술
immunoprecipitation (IP): 2.5-5.0 μg using lysates of transiently transfected cells expressing C-terminal-FLAG-tagged protein
western blot: 0.5-1.0 μg/mL using extracts of transiently transfected cells expressing C-terminal-FLAG-tagged protein
동형
IgG1
면역원 서열
(DYKDDDDK)
배송 상태
dry ice
저장 온도
−20°C
1 of 4
이 품목 | F7425 | SAB4200119 | F2555 |
|---|---|---|---|
| species reactivity all | species reactivity all | species reactivity all | species reactivity - |
| conjugate unconjugated | conjugate unconjugated | conjugate peroxidase conjugate | conjugate unconjugated |
| antibody form purified from hybridoma cell culture, purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form ascites fluid |
| clone 6F7, monoclonal | clone polyclonal | clone 6F7, monoclonal | clone SIG1-25, monoclonal |
| biological source rat | biological source rabbit | biological source rat | biological source rabbit |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
일반 설명
Monoclonal Anti-FLAG® (rat IgG1 isotype) is derived from the hybridoma 6F7 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG® peptide. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Monoclonal Anti-FLAG® recognizes N-terminal,
C-terminal and internal Flag-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG®-tagged fusion proteins.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in associationith other tags.[1] The small size of the FLAG® tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function.
The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage of the C-terminal FLAG® peptide from a fusion protein catalyzed by Cu2+ ions has been reported.[2] A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+dependent protein b-phosphatase[3], as well as in the human and bovine enzyme.
Monoclonal Anti-FLAG® recognizes N-terminal,
C-terminal and internal Flag-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG®-tagged fusion proteins.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in associationith other tags.[1] The small size of the FLAG® tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function.
The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage of the C-terminal FLAG® peptide from a fusion protein catalyzed by Cu2+ ions has been reported.[2] A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+dependent protein b-phosphatase[3], as well as in the human and bovine enzyme.
면역원
FLAG peptide
DYKDDDDK
DYKDDDDK
애플리케이션
ANTI-FLAG® antibody, Rat monoclonal has been used in:
- chromatin immunoprecipitation (ChIP)[4]
- western blotting[5]
- coimmunoprecipitation[6][7]
- flow cytometric analysis[7]
Browse additional application references in our FLAG® Literature portal.
Learn more product details in our FLAG® application portal.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
법적 정보
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wntbeta-catenin signaling pathway
Li Q, et al
Journal of Experimental & Clinical Cancer Research, 36(1), 85-85 (2017)
Rahul Bhowmick et al.
The Journal of biological chemistry, 287(42), 35004-35020 (2012-08-14)
Viruses have evolved to encode multifunctional proteins to control the intricate cellular signaling pathways by using very few viral proteins. Rotavirus is known to express six nonstructural and six structural proteins. Among them, NSP4 is the enterotoxin, known to disrupt
Xiaolei Li et al.
eLife, 6 (2017-08-19)
Acquired therapeutic resistance by tumors is a substantial impediment to reducing the morbidity and mortality that are attributable to human malignancies. The mechanisms responsible for the dramatic shift between chemosensitivity and chemoresistance in colorectal carcinoma have not been defined. Here
Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis
Dai L, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 99(6), 736-736 (2019)
Qing Deng et al.
Cell death & disease, 9(11), 1049-1049 (2018-10-17)
PHD finger protein 19 (PHF19), a critical component of the polycomb repressive complex 2 (PRC2), is crucial for maintaining the repressive transcriptional activity of several developmental regulatory genes and plays essential roles in various biological processes. Abnormal expression of PHF19
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