Yes. It is Ficoll 400. This product is graded molecular biology grade and is free of any nucleases
추천 제품
일반 설명
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
애플리케이션
Suitable for
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane[1]
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.[2]
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).[3]
Used for cell separation and organelle isolation.
특징 및 장점
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
기타 정보
A nonionic synthetic polymer of sucrose.
법적 정보
Ficoll is a registered trademark of Cytiva
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Optimization of isolated perfused/ventilated mouse lung to study hypoxic pulmonary vasoconstriction.
Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Cristina Batlle et al.
Cell reports, 30(4), 1117-1128 (2020-01-30)
Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been
Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
Bohan Zhang et al.
Life sciences, 258, 118145-118145 (2020-07-28)
Studies on diabetes mellitus have shown that the phosphoinositide 3-kinase (PI3K)/serine threonine kinase (AKT)/forkhead box protein O1 (FoxO1) signaling pathway can regulate insulin secretion by modulating the expression of pancreatic and duodenal homeobox-1 (PDX-1). Therefore, it was hypothesized that the
문서
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
프로토콜
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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Is Product F2637, Ficoll, the same as Ficoll 400?
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What is the difference between Ficoll 400 and Ficoll PM 400?
1 답변-
The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you have a protocols for use of Product F2637, Ficoll, in various applications?
1 답변-
Please check the product information sheet for F4375 which has a detailed protocol.
도움이 되었습니까?
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Can you use Product F2637, Ficoll, to isolate mononuclear cells?
1 답변-
When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.
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Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?
1 답변-
This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock:0.25% bromophenol blue0.25% xylene cyanol FF15% Ficoll
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