This product has not been tested for any specific application, including in vivo use. Suitability would have to be determined experimentally by the end user. Historical information suggests that a concentration of 0.5% Evans Blue in PBS at pH 7.4 has been used successfully in staining tissue sections. This concentration may also be effective starting point for in vivo applications. Please refer to available literature for additional information.
모든 사진(4)
크기 선택
보기 변경
10 G
₩133,879
50 G
₩542,213
100 G
₩867,521
About This Item
실험식(Hill 표기법):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
색상 지수 번호:
23860
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47
추천 제품
제품명
Evans Blue, Dye content ≥75 %
양식
powder
Quality Level
구성
Dye content, ≥75%
기술
titration: suitable
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
InChI key
ATNOAWAQFYGAOY-GPTZEZBUSA-J
유전자 정보
human ... PTPN1(5770) , PTPRF(5792)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.
애플리케이션
Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.
생화학적/생리학적 작용
Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability.[1] It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.[2]
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Carc. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Peterson RL, et al.
Teaching Plant Anatomy Through Creative Laboratory Exercises (2008)
CO2 is the main inorganic C species entering photosynthetically active leaf protoplasts of the freshwater macrophyte Ranunculus penicillatus ssp. pseudofluitans.
Plant, Cell and Environment, 22, 1019-1026 (1999)
Theodora Agalioti et al.
Nature communications, 8, 15205-15205 (2017-05-17)
Malignant pleural effusion (MPE) is the lethal consequence of various human cancers metastatic to the pleural cavity. However, the mechanisms responsible for the development of MPE are still obscure. Here we show that mutant KRAS is important for MPE induction
Brian P Eliceiri et al.
Methods in molecular biology (Clifton, N.J.), 686, 371-378 (2010-11-18)
The blood-brain barrier (BBB) is a monolayer of endothelial cells that is regulated by the proximity of a unique basement membrane and a tightly controlled molecular interaction between specialized subsets of cells including pericytes, astrocytes, and neurons. Working together, these
Diego Castanares-Zapatero et al.
Critical care medicine, 41(12), e411-e422 (2013-08-22)
As adenosine monophosphate (AMP)-activated protein kinase both controls cytoskeleton organization in endothelial cells and exerts anti-inflammatory effects, we here postulated that it could influence vascular permeability and inflammation, thereby counteracting cardiac wall edema during sepsis. Controlled animal study. University research
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What amount and concentration should I use Evans-Blaun injecting it into the ear skin of a mouse to see Evans-Blue in the draining Lymphnodes afterwards?
1 답변-
도움이 되었습니까?
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What is the stability of the E2129 solution once prepared?
1 답변-
The stability of the solution will depend on whether the solutions are sterile filtered or contaminated through routine use. It's recommended to store the solutions in the dark at 2-8°C.
도움이 되었습니까?
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How to prepare a stock 1% solution using E2129, and is ther any guidance on its use?
1 답변-
A 0.5% solution of Evans Blue in PBS, pH 7.4. Product P3813 should be used as the PBS, which will serve as the stock solution. For use, 3-4 drops should be added to 100 mL of PBS P3813. This should be used as the final rinse with a 5-minute wash. After rinsing briefly, coverslip the sample.
도움이 되었습니까?
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Should Evans Blue solutions for fluorescent assays be aged or cured for 7 days before use?
1 답변-
While some dyes require aging before use, there is no indication that Evans Blue solutions need to be aged or cured before use.
도움이 되었습니까?
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How do I dilute 80% purity evans blue powder into a 2% solution with normal saline?
1 답변-
This product is soluble in water at 60 mg/mL. For typical laboratory staining applications, a 1X PBS solution is also commonly used. Solubility in normal saline has not been evaluated, but rates are expected to be similar. A 2% solution would be 20 mg/mL. The dye content is lot-specific and reported in the Certificate of Analysis. Please see the link below to access a sample or batch-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/e2129#product-documentation도움이 되었습니까?
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What is the difference between Evans Blue Products E2129 and 206334?
1 답변-
Both products are Evans Blue. Product No. E2129 has a dye content of 80% and Product No. 206334 has a dye content of approximately 85%.
도움이 되었습니까?
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How should Product E2129, Evans Blue, be dissolved?
1 답변-
This product is soluble in water, alcohol, acids and alkalies.
도움이 되었습니까?
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Can Evans Blue, Product E2129, be used as a counterstain for FITC?
1 답변-
This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988).
도움이 되었습니까?
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What is the shelf life of Product E2129, Evans Blue?
1 답변-
This product has a retest date of 4 years. This information can be found on the Certificate of Analysis.
도움이 되었습니까?
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What is the solution stability of Product E2129, Evans Blue?
1 답변-
This product is rather stable in aqueous solution and may be autoclaved at 15 psi for 30 min. However, dye made up in physiological saline should not be autoclaved.
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