Dye content is determined by back calculation from the carbon or nitrogen content, while purity is established by HPLC. The dye content is 60%. The impurities are not identified by either dye content or by methods such as HPLC, and no testing for impurities is conducted in this case. Both dye content and dye purity are determined for this product, with the Specification sheet listing minimum specifications for all lots, and individual Certificates of Analysis reporting lot-specific results. Water content is not measured, and the product is not considered hygroscopic.
모든 사진(3)
크기 선택
보기 변경
25 G
₩221,067
100 G
₩704,918
About This Item
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
Molecular Weight:
960.81
색상 지수 번호:
23850
Beilstein:
4360496
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47
추천 제품
Quality Level
분석
≥80% (HPLC)
양식
powder
구성
Dye content, 60%
기술
microbe id | staining: suitable
색상
dark green to brown, or Brown-Black
mp
>300 °C (lit.)
solubility
H2O: 1 mg/mL
λmax
607 nm
ε (흡광계수)
≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
유전자 정보
human ... PTPN1(5770) , PTPRF(5792)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.
애플리케이션
생화학적/생리학적 작용
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
- to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
- to stain plant tissue in order to visualize cell death.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Carc. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
개인 보호 장비
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
이미 열람한 고객
Role of caspase-10 in the death of acute leukemia cells
Guo W
Oncology Letters, 12, 1623-1629 (2016)
Lack of Bax Prevents Influenza A Virus-Induced Apoptosis and Causes Diminished Viral Replication
McLean J E, et.al.
Journal of Virology, 83, 8233-8246 (2009)
Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 239, 698-704 (2001)
Thangaraj Parimelazhagan
Pharmacological Assays of Plant-Based Natural Products (2013)
Jeffrey E McLean et al.
Journal of virology, 83(16), 8233-8246 (2009-06-06)
The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic
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Are the descriptions of Product 302643 as "Trypan Blue - 60% Dye content" and the purity limit of ">= 80.0%" on the Specification sheet and CofA referring to the same thing? If they are, why are they different? If not, should both be considered when aiming to achieve a specific dye concentration? Additionally, if it is 60% dye, what makes up the remaining 40%? Should the significant water content in this product be taken into consideration?
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