This product is assigned a minimum activity specification of 2000 units per milligram protein and a minimum protein specification of 80%. At the minimum value for each specification, the 150,000 unit package size would contain 93.75 mg of powder using the following calculation:
Units/(activity x %protein) = mass or 150,000/(2000 x 0.80) = 93.75 mg
The activity and protein content are lot-specific and reported in the product Certificate of Analysis. As the activity increases the mass per package size decreases. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d5025#product-documentation
D5025
Deoxyribonuclease I from bovine pancreas
Type IV, lyophilized powder, ≥2,000 Kunitz units/mg protein
동의어(들):
DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
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크기 선택
15000 UNITS
₩203,812
150000 UNITS
₩725,522
375000 UNITS
₩1,496,415
750000 UNITS
₩2,329,222
모든 사진(5)
크기 선택
보기 변경
15000 UNITS
₩203,812
150000 UNITS
₩725,522
375000 UNITS
₩1,496,415
750000 UNITS
₩2,329,222
About This Item
추천 제품
생물학적 소스
bovine pancreas
유형
Type IV
양식
lyophilized powder
특이 활성도
≥2,000 Kunitz units/mg protein
분자량
~31 kDa
정제법
chromatography
구성
Protein, ≥80%
기술
DNA purification: suitable
solubility
0.15 M NaCl: soluble 5.0 mg/mL, clear, colorless
적합성
suitable for molecular biology
응용 분야
diagnostic assay manufacturing
diagnostic assay manufacturing
외래 활성
Chymotrypsin ≤0.5%
Protease ≤0.05%
RNase ≤0.02%
배송 상태
wet ice
저장 온도
−20°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
애플리케이션
DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the processing of rat brain tissue. This study showed that axonal growth on astrocytes is not inhibited by oligodendrocytes.[1] In another study, thawed fixed samples of E. coli were digested with DNAse I from Sigma along with other enzymes. The digestion was done before permeabilization and staining of the nucleic acids.[2]
Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
Used for the removal of DNA from protein samples.
생화학적/생리학적 작용
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+.[3] The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme.[4] A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found.[5] 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)[6] and actin[7] are known to inhibit the enzyme activity.
단위 정의
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate.
물리적 형태
Lyophilized powder containing calcium chloride
제조 메모
10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.
분석 메모
Protein determined by biuret.
저해제
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
T Guindulain et al.
Applied and environmental microbiology, 63(11), 4608-4611 (1997-11-15)
Three nucleic acid dyes (SYTO-13, TOTO-1, and YOYO-1) were tested on cultures of Escherichia coli and marine prokaryote populations. These dyes stain the RNA and DNA in E. coli but only respond to DNA in marine populations, according to the
J W Fawcett et al.
Journal of cell science, 103 ( Pt 2), 571-579 (1992-10-01)
Axon growth in vitro may be inhibited by contact with oligodendrocytes, but most axons grow readily on the surface of astrocyte monolayers. Since both cell types are in close contact with one another in the damaged nervous system, we have
Enzymes of Molecular Biology
Weir, A. F.
Methods in Molecular Biology, 16 (1993)
Chii J Chan et al.
Biophysical journal, 112(6), 1063-1076 (2017-03-30)
Understanding the physical mechanisms governing nuclear mechanics is important as it can impact gene expression and development. However, how cell nuclei respond to external cues such as heat is not well understood. Here, we studied the material properties of isolated
Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 2(2), 5-5 (2001)
프로토콜
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.
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How many milligrams are in one vial of D5025-150KU?
1 답변-
도움이 되었습니까?
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Good afternoon, if the stock concentration of 10mg/ml may loose 10% of its activity after one week, I was wondering if it is okey regarding stability to keep 500ug/ml aliquots at -20ºC for several weeks.
1 답변-
As cited in the product listing and datasheet, this product in solution shows an approximate loss of activity of 10% when stored in aliquots at −20 °C for 1 week. Studies at lower dilutions have not been performed. Typically, solutions stored at higher concentrations are the most stable.
도움이 되었습니까?
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Is Kunitz unit same as unit here? I have seen some papers and they have U/ml for DNase. How can i conver it?
1 답변-
Product D5025, Deoxyribonuclease I is given in Kunitz units. This product is a powder. When reconstituting the product with a solution, for example 15,000 Kunitz units in 1 ml of 0.15 M NaCl the end-user will have 15,000 Kunitz units/ml.
도움이 되었습니까?
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How do Deoxyribonuclease I (DNase I), products D5025 and D4527 differ from each other?
1 답변-
Both of these products have the same specific activity (>2000 units/mg protein) for Dnase I. The enzyme impurity levels for each, however, are different. D5025 has the following impurity specification: Chymotrypsin (<0.5%), Protease (<0.05%), and RNase (<0.02%). The impurity specifications for D4527 are: Chymotrypsin (<0.01%), Protease (<0.005%), and RNase (<0.01%).
도움이 되었습니까?
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Are stock solutions of Product D5025, Deoxyribonuclease I (DNase I), stable?
1 답변-
Solutions of DNAse I (10 mg/ml) in 0.15 M NaCl may lose 10% of its activity when stored for a week in aliquots at -20°C. The same solutions stored in aliquots at 2 - 8°C. lose approximately 20% activity.
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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How can I convert units of Product D5025, Deoxyribonuclease I (DNase I), to mass?
1 답변-
The mg of solid for each unit package size will be indicated on the label of the package. For a particular lot, you can calculate the specific activity in units per mg solid by multiplying the specific activity in units per mg protein by the percent protein divided by 100.
도움이 되었습니까?
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How should I prepare a solution of Product D5025, DNAse I?
1 답변-
This enzyme can be reconstituted in 0.15 M NaCl at a concentration of 10 mg/ml.
도움이 되었습니까?
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What concentration of Product D5025, Deoxyribonuclease I (DNase I), is required to remove DNA from solutions?
1 답변-
DNA can be removed from preparations by incubation with 20 - 50 ug DNAse I in the presence of 50 mM Tris-HCl buffer, pH 7.5, 10 mM magnesium chloride, at 37°C. for 60 min.
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