D5319

Deoxyribonuclease I bovine

recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein

• 동의어(들): DNAse I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
• 효소 위원회 번호: 3.1.21.1 (BRENDA, IUBMB)
• MDL number: MFCD00130918
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 재조합: expressed in Pichia pastoris
• 분석: ≥95%
• 양식: buffered aqueous glycerol solution
• 특이 활성도: ≥5,000 units/mg protein
• 분자량: ~39 kDa
• 기술: DNA extraction: suitable
• 적합성: suitable for molecular biology
• 응용 분야: diagnostic assay manufacturing
• 외래 활성: RNAse and protease, free
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

애플리케이션

DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study.[1] The enzyme has also been for the preparation and harvest of mice mammary glands.[2]

Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.

Used for the removal of DNA from protein samples.

생화학적/생리학적 작용

DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn²⁺, Ca²⁺, Co²⁺, and Zn²⁺ are activators of the enzyme. A concentration of 5 mM Ca²⁺ stabilizes the enzyme against proteolytic digestion.[3] 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)[4] and actin[5] are known to inhibit the enzyme activity.

Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

특징 및 장점

• RNA purification by removing DNA
• Prepare DNA for nick translation¹
• Footprinting assays to determine DNA-protein interactions²

단위 정의

One unit will produce a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C

물리적 형태

Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol

제조 메모

Produced without using any animal cells or animal derived materials.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable