CHP2

Imprint^® Ultra Chromatin Immunoprecipitation Kit

Complete ChIP kit for sensitivity, compatibility with Next-Gen sequencing

• UNSPSC 코드: 12352202

설명

일반 설명

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 10⁶ cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 10⁸ cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

애플리케이션

• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

특징 및 장점

• Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
• Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 10⁶
• Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 10⁶ cells
• Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

법적 정보

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

키트 구성품 전용

• Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
• Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

키트 구성품 역시 별도로 이용 가능함

• S6576 ChIP Next Gen Seq Sepharose^™
• I8896 IGEPAL^® CA-630, for molecular biology
• S5150 Sodium chloride solution, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture
• W4502 Water, Nuclease-Free Water, for Molecular Biology
• I5006 IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
• I5381 IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
• A7638 Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
A8456 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization
M7023 Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
R4642 Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

모두 보기 (12)

안전성 정보

• 픽토그램: GHS02,GHS03,GHS08,GHS05,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H226,H271,H290,H302,H314,H334,H336,H410
• 예방조치 성명서: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3
• 표적 기관: Central nervous system
• Storage Class Code: 5.1A - Strongly oxidizing hazardous materials