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Merck
모든 사진(1)

문서

NUC101

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

동의어(들):

Rapid mammalian nuclei isolation kit

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.32

사용

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

유통기한

1 yr at 2‑8 °C

포장

pkg of 1 kit

응용 분야

cell analysis

외래 활성

nuclease and protease, free

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

애플리케이션

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

생화학적/생리학적 작용

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

기타 정보

All procedures should be carried out on ice or 2-8°C.

관련 제품

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
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Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
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A major goal of post-genomics research is the integrated analysis of genes, regulatory elements and the chromatin architecture on a genome-wide scale. Mapping DNase I hypersensitive sites within the nuclear chromatin is a powerful and well-established method of identifying regulatory
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문서

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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