추천 제품
사용
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
유통기한
1 yr at 2‑8 °C
포장
pkg of 1 kit
응용 분야
cell analysis
외래 활성
nuclease and protease, free
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.
애플리케이션
Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.
생화학적/생리학적 작용
The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
기타 정보
All procedures should be carried out on ice or 2-8°C.
관련 제품
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
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Analysis of nuclear RNA, Chapter 14.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
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문서
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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