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Merck
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주요 문서

Hep G2 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 85011430, human liver, Epithelial

동의어(들):

HepG2 Cells

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모든 사진(1)

About This Item

UNSPSC 코드:
41106514
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생물학적 소스

human liver

포장

tube of 5 μg 85011430-DNA-5UG
vial of  cells 85011430-1VL

성장 모드

Adherent

핵형

Modal no. 55

형태학

Epithelial

제품

Prothrombin, antithromin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen

수용체

Not specified

기술

cell culture | mammalian: suitable

관련 질환(들)

cancer

배송 상태

dry ice

저장 온도

−196°C

관련 카테고리

세포주 기원

Human Caucasian hepatocyte carcinoma

세포주 설명

The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.

애플리케이션

Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay.[1][2]
Study of hepatocyte function and specific protein expression

포장

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10
THO1: 9
TPOX: 8,9
vWA: 17

배양 배지

EMEM (EBSS)+ 2mM Glutamine + 1% Non Essential Amino Acids NEAA) + 10% Foetal Bovine Serum FBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells might take 6 days to form a confluent monolayer without media change if seeded at 1:4. Until then growth occurs mainly in islands.

기타 정보

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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

질문

1–7 / 7 질문  

  1. Are my Cell Line cells mycoplasma tested?

    1 답변

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  2. Are the Cell Line cells tested for viral pathogens?

    1 답변

    1. HPA Cultures does not perform any viral testing on the cell lines.

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  3. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 답변

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

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  4. What passage are my Cell Line cells?

    1 답변

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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  5. How many Cell Line cells are in the vial?

    1 답변

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  6. What medium should I use when I thaw the Cell Line cells?

    1 답변

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  7. How do I handle Cell Line frozen cells upon arrival?

    1 답변

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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