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Merck
모든 사진(4)

문서

A6106

Sigma-Aldrich

Aprotinin

BioUltra, 3-8 TIU/mg solid, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

동의어(들):

BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)

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모든 사진(4)

About This Item

실험식(Hill 표기법):
C284H432N84O79S7
CAS Number:
9087-70-1
Molecular Weight:
6511.44
EC Number:
232-994-9
MDL number:
MFCD00130541
UNSPSC 코드:
12352204
NACRES:
NA.77

product name

Aprotinin from bovine lung, BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)

생물학적 소스

bovine lung

제품 라인

BioUltra

분석

≥98% (SDS-PAGE)

형태

powder

특이 활성도

3-8 TIU/mg solid

분자량

~6,500

기술

inhibition assay: suitable

solubility

H2O: 5 mg/mL, clear to very slightly hazy

UniProt 수납 번호

P00974

저장 온도

2-8°C

InChI key

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

유전자 정보

cow ... PTI(404172)

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관련 카테고리

애플리케이션

Aprotinin is largely used as an inhibitor of trypsin.

생화학적/생리학적 작용

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

단위 정의

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

제조 메모

A further purification of A1153 to yield a product with slightly higher activity and purity

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

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문서

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

프로토콜

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

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