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Merck
모든 사진(1)

주요 문서

11426320001

Roche

Anti-Fluorescein

from mouse IgG1 (clone B13-DE1)

동의어(들):

antibody

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About This Item

UNSPSC 코드:
12352200

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

B13-DE1, monoclonal

양식

lyophilized

포장

pkg of 100 μg

제조업체/상표

Roche

동형

IgG1

저장 온도

2-8°C

일반 설명

Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.

Contents
Lyophilizate, stabilized

특이성

The monoclonal antibody reacts with free and bound fluorescein.

애플리케이션

Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization[1]
  • Western blot
Anti-Fluorescein has been used to capture amplicon in quadruple-tagging PCR and nucleic acid lateral flow assay for simultaneous detection of foodborne pathogens.[2]

제조 메모

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.

재구성

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.

기타 정보

For life science research only. Not for use in diagnostic procedures.

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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


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시험 성적서(COA)

Lot/Batch Number

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