추천 제품
생물학적 소스
sheep
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
양식
lyophilized
포장
pkg of 200 μg
제조업체/상표
Roche
동형
IgG
저장 온도
2-8°C
일반 설명
Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.[1]
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.[2]
특이성
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
애플리케이션
생화학적/생리학적 작용
In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.[7]
제조 메모
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.
재구성
Add 1 ml PBS to a final concentration of 200 μg/ml.
분석 메모
No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.
기타 정보
For life science research only. Not for use in diagnostic procedures.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
이미 열람한 고객
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PloS one, 7(5), e38156-e38156 (2012-06-14)
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We describe a proof-of-principle, immune sandwich assay in which immune complexes link micron-size beads via DNA tethers to a sensor surface. The number of tethered beads, counted using low-magnification microscopy, provides a measure of the concentration of analyte. The prototype
문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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