추천 제품
생물학적 소스
sheep
Quality Level
결합
alkaline phosphatase conjugate
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
양식
solution
포장
pkg of 150 U (200 μl)
제조업체/상표
Roche
동형
IgG
저장 온도
2-8°C
일반 설명
Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions.[1] Fab fragments bind to G proteins.[2]
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)
특이성
The polyclonal antibody reacts with free and bound fluorescein.
애플리케이션
Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
- Dot blot
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Southern blot
- Western blot
- Electrochemical biosensing technique for the detection of 16S rRNA
생화학적/생리학적 작용
Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).[3]
특징 및 장점
Contents
Solution, stabilized
Solution, stabilized
제조 메모
- Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
- ELISA: 150 to 300mU/ml
- Immunohistocytochemistry: 250 to 500mU/ml
- In situ hybridization: 1.5 to 7.5U/ml
- Southern blot: 150mU/ml
- Western blot: 250 to 500 mU/ml
Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
기타 정보
For life science research only. Not for use in diagnostic procedures.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
WGK
WGK 1
Flash Point (°F)
No data available
Flash Point (°C)
No data available
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