431525

L-Leucine Dehydrogenase, Bacillus cereus

• 동의어(들): L-Leucine Dehydrogenase, Bacillus cereus, L-LeuDH, L-Leucine:NAD ⁺-oxidoreductase
• CAS Number: 9082-71-7
• 효소 위원회 번호: 1.4.1.9 (BRENDA, IUBMB)
• MDL number: MFCD00147461
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: Bacillus sp. (Bacillus cereus)
• Quality Level: 100
• 양식: liquid
• 특이 활성도: ≥10 units/mg protein; ≥50 units/mL
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.

pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD⁺ per min at 30°C, pH 10.7.

물리적 형태

In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.

재구성

Following initial thaw, aliquot and freeze (-20°C).

기타 정보

Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2