G2626

L-Glutamic Dehydrogenase from bovine liver

Type II, 50% glycerol solution, ≥35 units/mg protein

• 동의어(들): L-GLDH, L-Glutamate:NAD[P]⁺ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver
• CAS Number: 9029-12-3
• 효소 위원회 번호: 1.4.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131461
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine liver
• Quality Level: 200
• 유형: Type II
• 양식: glycerol solution (50%)
• 특이 활성도: ≥35 units/mg protein
• 분자량: 310-350 kDa
• UniProt 수납 번호: P00366
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: cow ... GLUD1(281785)

설명

애플리케이션

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.[1]

생화학적/생리학적 작용

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:

• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

단위 정의

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

물리적 형태

50% glycerol solution

분석 메모

Protein determined by biuret.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable