324796

Endopeptidase Lys-C, Achromobacter lyticus

• 동의어(들): Endopeptidase Lys-C, Achromobacter lyticus
• CAS Number: 78642-25-8
• MDL number: MFCD00130333
• UNSPSC 코드: 12352202

속성

• 형태: lyophilized
• Quality Level: 100
• 특이 활성도: ≥2 AU/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 기술: mass spectrometry (MS): suitable
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues.

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. Optimal pH: 9.0-9.5.

경고

Toxicity: Standard Handling (A)

단위 정의

One AU (amidase unit) is defined as the amount of enzyme that catalyzes the release of 1 µM pNA from N-benzoyl-DL-lysine-pNA per min at 30°C, pH 9.5.

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Reconstitute 1 vial with 1 ml solvent; if the enzyme is not completely solubilized, add 1-3 ml additional solvent until completely dissolved.

기타 정보

Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany