The product has a solubility of 25 mg/mL in acetonitrile and is also soluble in DMF and DMSO. After reconstitution, the stock can be diluted in a buffer or medium. Please note that it is not a sterile product, so sterile filtration is necessary for stock solutions. It is advisable to store the solution in single-use aliquots for up to 1 week at -20°C, protected from light.
216254
BCECF/AM
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.
동의어(들):
BCECF/AM, 2ʹ,7ʹ- bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester, 2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester
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크기 선택
1 MG
₩259,231
₩259,231
예상 입고일2025년 4월 03일세부사항
모든 사진(1)
크기 선택
보기 변경
1 MG
₩259,231
About This Item
₩259,231
예상 입고일2025년 4월 03일세부사항
추천 제품
Quality Level
분석
≥85% (mixture of isomers, HPLC)
양식
solid
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
protect from light
색상
white to yellow
solubility
acetonitrile: 25 mg/mL
DMF: soluble
DMSO: soluble
형광
λex 500-504 nm
λem 525-531 nm
배송 상태
ambient
저장 온도
−20°C
SMILES string
O1C3(c4c(cc(c(c4)CCC(=O)OCOC(=O)C)OCOC(=O)C)Oc5c3cc(c(c5)OCOC(=O)C)CCC(=O)OCOC(=O)C)c2c(cc(cc2)C(=O)OCOC(=O)C)C1=O
InChI key
NTECHUXHORNEGZ-UHFFFAOYSA-N
일반 설명
A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
생화학적/생리학적 작용
Cell permeable: yes
Primary Target
Monitor intracellular pH changes
Monitor intracellular pH changes
Product does not compete with ATP.
Reversible: no
포장
Packaged under inert gas
경고
Toxicity: Irritant (B)
재구성
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
기타 정보
Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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