While the specifications for this probe do not include assaying for specific applications, there are many publications showing its use in flow cytometry applications:
https://www.sciencedirect.com/science/article/pii/S0022030215000909
https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1002/iub.1895
https://www.tandfonline.com/doi/pdf/10.1080/21655979.2021.1994737
https://journal.waocp.org/article_91422_6ccd2a19eb9159073edeb8f9ce0be660.pdf
https://pmc.ncbi.nlm.nih.gov/articles/PMC7842666/
D6883
2′,7′-Dichlorodihydrofluorescein diacetate
≥97% (HPLC), powder, cell-permeable non-fluorescent probe
동의어(들):
2′,7′-Dichlorofluorescin diacetate, DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate
로그인조직 및 계약 가격 보기
모든 사진(2)
크기 선택
보기 변경
50 MG
₩111,752
250 MG
₩404,579
About This Item
실험식(Hill 표기법):
C24H16Cl2O7
CAS Number:
Molecular Weight:
487.29
Beilstein:
4610273
MDL number:
UNSPSC 코드:
12352200
PubChem Substance ID:
NACRES:
NA.77
추천 제품
제품명
2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%
Quality Level
분석
≥97%
양식
powder
저장 온도
−20°C
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.[1]
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.
애플리케이션
2′,7′-Dichlorofluorescin diacetate is suitable for use in:
- sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism[2][1][3]
- microplate assay for detecting oxidative products in phagocytic cells
- quantitative multiwell myeloid differentiation assay
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
저장 및 안정성
This product should be stored in powdered format at -20°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Yeonhwa Song et al.
Journal of experimental & clinical cancer research : CR, 36(1), 38-38 (2017-03-04)
CD133-positive cells in hepatocellular carcinoma (HCC) exhibit cancer stem cell (CSC)-like properties as well as resistance to chemotherapeutic agents and ionizing radiation; however, their function remains unknown. In this paper, we identified a hitherto unknown mechanism to overcome CD133-induced resistance
Katia Cortese et al.
Life sciences, 232, 116610-116610 (2019-06-30)
The aim of this study was the characterization of the in vitro cytotoxic properties of a recently isolated diterpene compound, 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone (compound 1), extracted from Salvia corrugata, versus human cell lines. We used as model study immortalized breast epithelial cells
Pornthip Chaichompoo et al.
Journal of clinical pathology, 72(8), 520-524 (2019-04-24)
Iron overload is a major factor contributing to the overall pathology of thalassaemia, which is primarily mediated by ineffective erythropoiesis and shorter mature red blood cell (RBC) survival. Iron accumulation in RBCs generates reactive oxygen species (ROS) that cause cellular
Yuan Ji et al.
Journal of agricultural and food chemistry, 67(32), 9060-9069 (2019-07-25)
Glutathione S-transferases (GSTs) play an active role in the development of drug resistance by numerous cancer cells, including melanoma cells, which is a major cause of chemotherapy failure. As part of our continuous effort to explore why dietary polyphenols bearing
Yilin Hong et al.
Life sciences, 231, 116550-116550 (2019-06-12)
Acrylamide (AA) is a common heat-generated toxicant in some food. Inhibiting its formation with natural antioxidants is of great significance. The current study aims to investigate the alleviative effect and the underlying mechanism of allicin against AA-induced oxidative stress in
문서
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
DISCOVER Bioactive Small Molecules for Nitric Oxide & Cell Stress Research
관련 콘텐츠
일산화질소 (NO) 및 세포 스트레스 연구를 위한 생체 활성 저분자
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Is D6883 2´,7´-Dichlorofluorescin diacetate suitable for flow cytometry analyses?
1 답변-
도움이 되었습니까?
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What is the protocol for measuring ROS using D6883? Specifically, what kind of media or buffer is used with this product?
1 답변-
The specific protocol for measuring ROS using D6883 and the type of media or buffer to be used with this product are not available. However, typically, this product is added to a buffer solution shortly before assessing the fluorescent signal. For further guidance, refer to the following citations for relevant information:
1. Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells
PMID: 261996772. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937
PMID: 2057064These publications may provide insights into the protocol for using D6883 and the type of media or buffer used with this product.
도움이 되었습니까?
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What concentration of DCF can I use for A549 cells?
1 답변-
This product has not been tested for use in cell culture. A search of the literature suggests working concentrations range from 5 µM to 0.1 mM. See the link below to a publication that specifically looked at A549 cells using several dyes, including DCFDA:
https://pubs.rsc.org/en/content/articlehtml/2017/ra/c7ra01025g도움이 되었습니까?
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What concentration of DCF can I use for THP-1 monocyte cell line?
1 답변-
The optimal concentration of 2′,7′-Dichlorofluorescin diacetate (DCFDA) for use with THP-1 monocytes will need to be determined by the end-user. See the link below to a paper that reported using DCF at 40 uM with THP-1 cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961584/도움이 되었습니까?
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Is it possible to view this probe under a fluorescence microscope if the probe is solubilised in DMSO? What are the excitation and absorption wavelengths?
1 답변-
The excitation wavelength is 504 nm and the emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however, various publications suggest that it is suitable. See the link below for an example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/도움이 되었습니까?
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Protocol please! Thank you very much!
1 답변-
It may be helpful to view the following articles as there is not a specific protocol for this product:
도움이 되었습니까?
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Can the product be stored in powder form and if its possible what is the storage temp?
1 답변-
This product should be stored in powdered format at -20°C.
도움이 되었습니까?
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What is the solution stability of Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 답변-
This product can be stored at -20°C for up to six months in solvents such as DMSO, ethanol, or DMF (N,N-dimethylformamide).
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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What is the excitation and emission wavelength for Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 답변-
The excitation wavelength is 504 nm and emission wavelength is 529 nm (in ethanol).
도움이 되었습니까?
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활성 필터
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