추천 제품
Grade
for fluorescence
제품 라인
BioReagent
양식
solid
solubility
DMF: soluble
DMSO: soluble
acetonitrile: soluble
형광
λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase)
적합성
suitable for fluorescence
저장 온도
2-8°C
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애플리케이션
An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues[1][2][3], intercellular spaces[4][5], mammalian cells[6][7][8], plant cells[9], bacteria[10][11], and yeast[12][13]. Also, employed to investigate in assays for cellular functional properties such as adhesion[14][15][16][17][18][19], multi-drug resistance[20][21][22], viability and cytotoxicity[23][24][25][26][27], apoptosis[28] and chemotaxis[15].
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
포장
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
H W van Veen et al.
The Journal of biological chemistry, 269(47), 29509-29514 (1994-11-25)
The strictly aerobic, polyphosphate-accumulating Acinetobacter johnsonii strain 210A degrades its polyphosphate when oxidative phosphorylation is impaired. The endproducts of this degradation, divalent metal ions and inorganic phosphate, are excreted as a neutral metal-phosphate (MeHPO4) chelate via the electrogenic MeHPO4/H+ symport
M T Alonso et al.
The Biochemical journal, 272(2), 435-443 (1990-12-01)
The effects of arachidonic acid and thrombin on calcium movements have been studied in fura-2-loaded platelets by a procedure which allows simultaneous monitoring of the uptake of manganese, a calcium surrogate for Ca2+ channels, and the release of Ca2+ from
R S Haworth et al.
Biochimica et biophysica acta, 1098(1), 79-89 (1991-12-13)
We have tested the efficacy of fluorescent probes for the measurement of intracellular pH in Saccharomyces cerevisiae. Of the compounds tested (fluorescein, carboxyseminaphthorhodafluor-1 (C.SNARF-1) and 2',7'bis(carboxyethyl)-5(6')-carboxyfluorescein), C.SNARF-1 was found to be the most useful indicator of internal pH. Fluorescence microscopy
P J Harris et al.
The American journal of physiology, 266(1 Pt 1), C73-C80 (1994-01-01)
The lateral intercellular spaces (LIS) of reabsorptive epithelia are the site of the proposed local osmotic gradient responsible for transepithelial transport. We developed techniques for loading the LIS of living cultured renal cells (MDCK and LLC-PK1) with the fluorescent dye
J T Seo et al.
Pflugers Archiv : European journal of physiology, 426(1-2), 75-82 (1994-01-01)
Intracellular pH (pHi) has been measured in intact, perfused rat mandibular salivary glands loaded with the fluorescent pH indicator BCECF [2',7'-bis(2-carboxyethyl)-5(6)- carboxyfluorescein]. Glands mounted in the cuvette of a conventional bench-top spectro-fluorometer were perfused for 5 min with the acetoxymethyl
문서
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
형광 수명 측정은 강도 기반 측정에 비해 유리한 점이 있습니다. 응용분야에는 형광 수명 분석, 감지 및 FLI가 포함됩니다.
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
