93595

Trypan Blue solution

0.4%, for microscopy

• 실험식(Hill 표기법): C₃₄H₂₄N₆Na₄O₁₄S₄
• CAS Number: 72-57-1
• Molecular Weight: 960.81
• Beilstein: 4360496
• MDL number: MFCD00003969
• UNSPSC 코드: 12171500
• PubChem Substance ID: 329770254
• NACRES: MA.02

속성

• Grade: for microscopy
• Quality Level: 200
• 형태: liquid
• 유통기한: limited shelf life, expiry date on the label
• 농도: 0.4%
• 색상: blue to very dark blue
• density: 1.007 g/mL at 20 °C
• εmax: 1.4 at 603 nm in methanol
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J

설명

일반 설명

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

애플리케이션

Trypan blue applications include:

• it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
• employed as a collagen stain in a Van Gieson procedure
• To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
• used as a fluorescent tracer of cell populations in embryology
• used as a tumor promoter modulating permeability of lysosomal membranes

Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

생화학적/생리학적 작용

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

물리적 형태

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H350
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type ABEK (EN14387) respirator filter