HPLCによるスパーオキシドジスムターゼの分析、Discovery^® BIO PolyMA-WAX、イオン交換

column

Discovery BIO PolyMA-WAX, 5 cm × 4.6 mm, 5 μm particles (59602-U)

mobile phase

([A] 20 mM Tris, pH 8.0 with hydrocloric acid; [B] 20 mM Tris, 0.5 M sodium chloride, pH 8.0 with hydrochloric acid)

gradient

0 to 10% B in 30 min. (linear)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μL