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HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series

HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series application for HPLC

材料

分析カラム

製品番号
詳細
価格

53970-U

Ascentis® Express HILIC、2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 10 cm × 3 mm

標準

製品番号
詳細
価格

S9378

スクロース

≥99.5% (GC)

T0625

タウリン

≥99%

1286504

フルクトース

United States Pharmacopeia (USP) Reference Standard

G0350500

グルコース

European Pharmacopoeia (EP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

dilute1:9 in acetonitrile

詳細

アナリシスノート

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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