HPLC Analysis of Protein Standards (1) on BIOshell A400 Protein C4
材料
Standard
製品番号
詳細
価格
ガードカートリッジ
製品番号
詳細
価格
BIOshell A400 Protein C4, 3.4 μm Guard Cartridge
3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 ea分析カラム
製品番号
詳細
価格
CONDITIONS
column
BIOshell A400 Protein C4, 10 cm X 2.1 mm, 3.4 μm particles (66825-U)
mobile phase
(A) 75:25, [0.1% TFA in water]:[0.1% TFA in acetonitrile] ; (B) 25:75, [0.1% TFA in water]:[0.1% TFA in acetonitrile]
gradient
25 to 65% B in 8 min
flow rate
0.3 mL/min
pressure
1430 psi (99 bar)
detector
UV, 215 nm
injection
1 μL
sample
protein mixture, g/L ea: lysozyme, 0.1; haptoglobulin, 0.3; conalbumin, 0.3; trypsin inhibitor, 0.3; glutamate dehydrogenase, 0.8; beta-galactosidase, 0.7; ovalbumin, 0.8; lactate dehydrogenase, 0.4
詳細
アナリシスノート
This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.
法的情報
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