HPLC Analysis of Organic Acids on Ascentis® RP-Amide
CONDITIONS
column
Ascentis RP-Amide, 10 cm x 2.1 mm I.D., 3 μm particles (565301-U)
mobile phase
[A] 0.1% (v/v) TFA in [B] water
flow rate
0.3 mL/min
column temp.
35 °C
detector
UV, 210 nm
injection
1 μL
sample
0.2 - 4 g/L in mobile phase
詳細
アナリシスノート
This application demonstrates the suitability of Ascentis RP-Amide for the analysis of organic acids under 100% aqueous conditions.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany