- Different co-culture systems have the same impact on bovine embryo transcriptome.
Different co-culture systems have the same impact on bovine embryo transcriptome.
Reproduction (Cambridge, England) (2017-10-07)
A Vitorino Carvalho, E Canon, L Jouneau, C Archilla, L Laffont, M Moroldo, S Ruffini, E Corbin, P Mermillod, V Duranthon
PMID28982934
要旨
During the last few years, several co-culture systems using either BOEC or VERO feeder cells have been developed to improve bovine embryo development and these systems give better results at high oxygen concentration (20%). In parallel, the SOF medium, used at 5% O
材料
製品番号
ブランド
製品内容
Sigma-Aldrich
MEM非必須アミノ酸溶液 (100X), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
ゲンタマイシン 溶液, 10 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
ウシ血清アルブミン ウシ血清由来, lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
199培地, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
BMEアミノ酸溶液 50×, Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
ヘパリン ナトリウム塩 from porcine intestinal mucosa, Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
199培地, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture