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Merck
  • Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.

Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.

Biomedical materials (Bristol, England) (2014-08-29)
Vadim L Zorin, Vladimir S Komlev, Alla I Zorina, Natalia V Khromova, Elena V Solovieva, Alexander Yu Fedotov, Ilya I Eremin, Pavel B Kopnin
要旨

Biocompatible ceramic fillers are capable of sustaining bone formation in the proper environment. The major drawback of these scaffolding materials is the absence of osteoinductivity. To overcome this limitation, bioengineered scaffolds combine osteoconductive components (biomaterials) with osteogenic features such as cells and growth factors. The bone marrow mesenchymal stromal cells (BMMSCs) and the β-tricalcium phosphate (β-TCP) are well-known and characterized in this regard. The present study was conducted to compare the properties of novel octacalcium phosphate ceramic (OCP) granules with β-TCP (Cerasorb(®)), gingiva-derived mesenchymal stromal cells (GMSCs) properties with the BMMSCs and osteogenic and angiogenic properties of a bioengineered composite based on OCP granules and the GMSCs. This study demonstrates that GMSCs and BMMSСs have a similar osteogenic capacity. The usage of OCP ceramic granules in combination with BMMSCs/GMSCs significantly affects the osteo- and angiogenesis in bone grafts of ectopic models.

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製品内容

Sigma-Aldrich
炭酸ナトリウム, powder, ≥99.5%, ACS reagent
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炭酸カリウム, ACS reagent, ≥99.0%
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デキサメタゾン, powder, BioReagent, suitable for cell culture, ≥97%
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炭酸ナトリウム, ACS reagent, anhydrous, ≥99.5%, powder or granules
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DAPI, for nucleic acid staining
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リン酸一アンモニウム, ACS reagent, ≥98%
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炭酸アンモニウム, ACS reagent, ≥30.0% NH3 basis
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炭酸カリウム, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99%
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炭酸ナトリウム, ACS reagent (primary standard), anhydrous, 99.95-100.05% dry basis
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炭酸カリウム, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)
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リノール酸, ≥98%
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酸化カルシウム, reagent grade
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炭酸ナトリウム, ReagentPlus®, ≥99.5%
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炭酸カリウム, reagent grade, ≥98%, powder, −325 mesh
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デキサメタゾン, ≥98% (HPLC), powder
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炭酸アンモニウム, 99.999% trace metals basis
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エチレンジアミン四酢酸 溶液, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
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炭酸ナトリウム, puriss., meets analytical specification of Ph. Eur., BP, NF, FCC, E500, anhydrous, 99.5-100.5% (calc. to the dried substance)
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インドメタシン, 98.5-100.5% (in accordance with EP)
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リン酸一アンモニウム, ReagentPlus®, ≥98.5%
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エチレンジアミン四酢酸, anhydrous, crystalline, BioReagent, suitable for cell culture
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リノール酸, liquid, BioReagent, suitable for cell culture
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エチレンジアミン四酢酸, 99.995% trace metals basis
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リン酸一アンモニウム, ≥99.99% trace metals basis
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フルオレセイン ジアセタート, used as cell viability stain
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炭酸ナトリウム, anhydrous, powder, 99.999% trace metals basis
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炭酸カリウム, ReagentPlus®, 99%
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酸化カルシウム, ReagentPlus®, 99.9% trace metals basis
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炭酸カリウム, 99.995% trace metals basis
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炭酸ナトリウム, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.5%