Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces.

Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces.

Current opinion in biotechnology (2013-04-02)

Leqian Liu, Heidi Redden, Hal S Alper

要旨

Microbial systems provide an attractive, renewable route to produce desired organic molecules such as fuels and chemicals. While attention within the field of metabolic engineering has mostly focused on Escherichia coli, yeast is a potent host and growing host for industrial products and has many outstanding, biotechnologically desirable native traits. Thus, there has been a recent shift in focus toward yeast as production hosts to replace E. coli. As such, products have diversified in yeast beyond simply ethanol. Additionally, nonconventional yeasts have been considered to move beyond Saccharomyces cerevisiae. This review highlights recent advances in metabolic engineering of yeasts for producing value-added chemical compounds including alcohols, sugar derivatives, organic acids, fats, terpenes, aromatics, and polyketides. Furthermore, we will also discuss the future direction of metabolic engineering of yeasts.

材料

製品番号

ブランド

製品内容

E7023

Sigma-Aldrich

エチルアルコール（純粋）, 200 proof, for molecular biology

09-0770

Sigma-Aldrich

エタノール, JIS special grade, ≥99.5%

362808

Sigma-Aldrich

Reagent Alcohol, reagent grade

09-0780

Sigma-Aldrich

エタノール, SAJ first grade, ≥99.5%

493511

Sigma-Aldrich

エチルアルコール（純粋）, 190 proof, ACS spectrophotometric grade, 95.0%

02857

Sigma-Aldrich

エタノール, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL¹, ~96% (based on denaturant-free substance)

E-029

Supelco

エタノール-50, 50 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

48075

Supelco

エタノール溶液, certified reference material, 2000 μg/mL in methanol

32221

Sigma-Aldrich

エタノール, puriss. p.a., absolute, ≥99.8% (GC)

E-030

Supelco

エタノール-80, 80 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-040

Supelco

エタノール-10, 10 mg/dL in H₂O, pack of 10 × 1.2 mL ampules, certified reference material, Cerilliant^®

E-053

Supelco

エタノール-500, 500 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-031

Supelco

エタノール-100 (10アンプル／キット), 100 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-033

Supelco

エタノール-300, 300 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E2385

Supelco

エタノール標準品10% (v/v), 10 % (v/v) in H₂O, analytical standard

E-041

Supelco

エタノール-150, 150 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

1012772

USP

エタノール, United States Pharmacopeia (USP) Reference Standard

09-0851

Sigma-Aldrich

エタノール, ≥99.5%, suitable for HPLC

E-032

Supelco

エタノール-200（10アンプル／キット）, 200 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

09-0790

Sigma-Aldrich

エタノール, JIS special grade, 94.8-95.8%

09-0852

Sigma-Aldrich

エタノール, ≥99.5%

E-056

Supelco

エタノール-20（10アンプル／キット）, 20 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-034

Supelco

Ethanol Calibration Kit, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-035

Supelco

エタノール溶液, 25 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-036

Supelco

エタノール-400（10アンプル／キット）, 400 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

187380

Sigma-Aldrich

エチルアルコール（変性）, reagent grade

09-0850

Sigma-Aldrich

エタノール, ≥99.5%, suitable for absorption spectrum analysis

2858

Sigma-Aldrich

Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)

09-0800

Sigma-Aldrich

エタノール, JIS first grade, 94.8-95.8%

E-045

Supelco

エタノール溶液, 40 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®