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  • Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome.

Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome.

Stem cell research (2020-09-12)
Morad Kamand, Mirolyuba Ilieva, Sheena Louise Forsberg, Mads Thomassen, Morten Meyer, Åsa Fex Svenningsen, Tanja Maria Michel
要旨

Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome ("FYNEN-cohort" of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

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ペニシリン-ストレプトマイシン, Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture
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ウシ血清アルブミン ウシ血清由来, heat shock fraction, pH 7, ≥98%
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Triton X-100, for molecular biology
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トリプシン-EDTA 溶液, 1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
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ジメチルスルホキシド, ≥99.5% (GC), suitable for plant cell culture
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中性緩衝ホルマリン溶液, 10%, histological tissue fixative
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2-メルカプトエタノール, ≥99.0%
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ゼラチン from porcine skin, gel strength 300, Type A
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