ZRB1875

Anti-Cleaved Caspase-8 Antibody, clone 1K3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: CASP-8, CASP8, EC: 3.4.22.61, FLICE, MACH, MCH5
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1K3, recombinant monoclonal
• 詳細: recombinant, expressed in HEK 293 cells
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 43 kDa; observed mol wt ~55 kDa
• 精製方法: using Protein A
• 化学種の反応性: human, mouse
• 包装: antibody small pack of 25
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: C-terminal half
• タンパク質IDアクセッション番号: NP_001073595
• UniProtアクセッション番号: O89110
• 環境により配慮した代替製品カテゴリ: Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• 遺伝子情報: mouse ... Casp8(12370)

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1K3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects cleaved form of Caspase-8. It targets an epitope within 23 amino acids from the cleavage site within the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 23 amino acids from the cleavage site within the C-terminal half of mouse Caspase-8.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HeLa treated with Staurosporin.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cleaved Caspase-8 in lysates from overnight serum starved HeLa cells treated with Staurosporin (0.5 M; 6 h).

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cleaved Caspase-8 in lysates from NIH3T3 cells treated with Etoposide (100 nM; 2 h).

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in Mouse brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in NIH3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Caspase-8 (UniProt: O89110; also known as EC: 3.4.22.61, CASP-8, CASP8, MCH5, FLICE, MACH) is encoded by the Casp8 gene (Gene ID: 12370) in murine species. Caspase-8 is a cysteine protease that serves as a molecular switch for apoptosis, necroptosis, and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. It is an initiator caspase that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. It is expressed in wide variety of tissues and its highest expression has been reported in spleen, thymus, lung, liver, and kidney. Low levels of expression are observed in heart, brain, testis, and skeletal muscle. Caspase-8 is produced with pro-peptides (aa 1-218 and 377-387) that are cleaved under appropriate signals to produce a p20 (aa 219-376) and a p10 (aa 388-480) subunits that heterotetramerize to form active caspase-8 with p20 and two p10 subunits. Generation of the subunits requires association with the death-inducing signaling complex (DISC). Activated caspase 8 is subsequently released to the cytosol where it cleaves downstream effector caspases. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Newton, K., et al. (2019). Nature. 574(7778); 428-431; Fritsch, M., et al. (2019). Nature. 575(7784); 683-687).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

再構成

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable