V9764

Anti-VPS34 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗ホスホイノシチド-3-キナーゼ抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~105 kDa
• 交差性: mouse, rat, human
• 濃度: ~1.0 mg/mL
• テクニック: indirect immunofluorescence: suitable; western blot: 2-4 μg/mL using whole extracts of HEK-293T cells expressing human VPS34
• UniProtアクセッション番号: Q8NEB9
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PIK3C3(5289) ; mouse ... Pik3c3(225326) ; rat ... Pik3c3(65052)

詳細

詳細

Vacuolar protein sorting 34 (VPS34), also known as phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), is encoded by the gene mapped to human chromosome 18q12.3. The encoded protein belongs to the phosphoinositide (PI)-3-kinase family. VPS34 is widely expressed in all tissues of mammals.

免疫原

synthetic peptide corresponding to amino acids 22-34 of human VPS34, conjugated to KLH via an added cysteine residue. The corresponding sequence is identical in mouse and rat.

アプリケーション

Anti-VPS34 antibody produced in rabbit has been used in immunoblotting.[1]

生物化学的／生理学的作用

Vacuolar protein sorting 34 (VPS34) is a lipid kinase that phosphorylate phosphatidylinositol (PtdIns), to form phosphatidylinositol 3 phosphate (PtdIns3P). VPS34 plays a vital role in various intracellular trafficking events such as autophagy and phagosome formation. Thus, the inhibition of kinase activity of Vps34 might be considered as a potential therapeutic method for cancer treatment. VPS34 is also involved in internal vesicle formation within multivesicular endosomes, reverse transport of endosome to Golgi apparatus and membrane transport from the outer nuclear membrane towards the cell periphery. Mutation in the gene encoding VPS34 is associated with pathogenesis of schizophrenia. VPS34 plays an essential role in nutrient/amino acid induced activation of mechanistic target of rapamycin (mTOR) signaling in cultured cells and it also facilitates early embryogenesis and cell proliferation.

ターゲットの説明

VPS34 is known to be involved in endocytosis and vesiculartrafficking.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)