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由来生物
human
リコンビナント
expressed in CHO cells
アッセイ
≥95% (HPLC)
≥95% (SDS-PAGE)
形状
lyophilized
分子量
24.4 kDa
包装
pkg of 20 μg
不純物
endotoxin, tested
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
遺伝子情報
human ... RSPO2(340419)
関連するカテゴリー
詳細
Recombinant human R-Spondin-2 (R-Spo2) is a 24.4kDa protein consisting of 212 amino acid residues. R-Spo2 is encoded by the gene mapped to human chromosome 8q23.1. The encoded protein belongs to the R-spondin family of secreted proteins. Members of RSpo family contain two furin-like cysteine-rich domains at N- terminal end and thrombospondin structural domain and a basic charged C- terminal domain.
生物化学的／生理学的作用
R-Spondin 2 (R-Spo2) plays a vital role in the activation of canonical Wnt signaling pathway, which is essential for normal morphogenesis of the respiratory tract and limbs. R-Spo2 positively regulates Wnt-dependent mineralization of osteoblasts, myogenic differentiation and hypertrophic myotube formation. R-Spo2 is also involved in skeletal development. Aberration in the expression of RSPO2 causes ossification of the posterior longitudinal ligament of the spine (OPLL), resulted by the altered differentiation of spinal ligament MSCs (mesenchymal stem cells) into chondrocytes. R-Spo2 is associated with various developmental processes, such as myogenesis, craniofacial morphogenesis, keratinocyte proliferation, osteoblast maturation, lung and limb development. R-Spo2 interacts with leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5) and stimulates acetylcholine receptor (AChR) clustering at the neuromuscular junction (NMJ).
物理的形状
Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.
再構成
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SRP3322-20UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis.
Bell SM
Development, 135(6), 1049-1058 (2008)
R-Spondin family members regulate the Wnt pathway by a common mechanism.
Kim KA
Molecular Biology of the Cell, 19(6), 2588-2596 (2008)
Secreted factor R-Spondin 2 is involved in refinement of patterning of the mammalian cochlea.
Mulvaney JF
Developmental Dynamics, 242(2), 179-188 (2013)
R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5.
Nakashima H
Scientific Reports, 6:28512 (2016)
Minke E Binnerts et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(37), 14700-14705 (2007-09-07)
The R-Spondin (RSpo) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. We have previously shown that RSpo proteins can induce proliferative effects on the gastrointestinal epithelium in mice. Here we provide a mechanism whereby RSpo1
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