SRP3070

IGF-II ヒト

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 別名: インスリン様増殖因子-II
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥98% (HPLC); ≥98% (SDS-PAGE)
• フォーム: lyophilized
• 有効性: ≤2.0 ng/mL ED₅₀
• 分子量: 7.5 kDa
• 包装: pkg of 50 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.1 EU/μg endotoxin, tested
• 色: off-white to yellow
• UniProtアクセッション番号: P01344
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... IGF2(3481)

詳細

詳細

Insulin-like growth factors (IGFs) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the insulin gene family, which also contains insulin and relaxin.[1] The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR),[2] but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.

アプリケーション

IL-4 from mouse has been used to activate B-cells.[3]

生物化学的／生理学的作用

The insulin-like growth factors (IGFs) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro.[1]

シーケンス

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

物理的形状

凍結乾燥品(添加物不含)。

再構成

バイアルは、開封前に遠心してください。水で濃度0.1～1.0 mg/mLに復元してください。ボルテックスは行なわないでください。水溶液は2～8°Cで最長1週間保存できます。長期保存には、担体タンパク質(0.1% BSAなど)含有バッファーでさらに希釈し、1回の使用量ずつに分注して-20～-80°Cで保存することを推奨します。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable